Integration of electrokinetic enrichment of spike protein with a lateral flow assay

Here, we present a SARS-CoV-2 spike (S) protein lateral flow assay enhanced by electrokinetic focusing driven by ion concentration polarization (ICP). In this study, we optimize the configuration of membrane-modified electrodes for a stable location and greatest degree of enrichment for the plug of S-protein. We also show that a test line comprising bioconjugated beads further enhances enrichment. Using this system, we have achieved 40X enrichment of S-protein. We are currently evaluating the impact of increased dwell time over the test line. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

AN INTEGRATED RT-LAMP AND CRISPR ASSAY FOR NUCLEIC ACID DETECTION IN A SINGLE MICROFLUIDIC CHIP

We will present a microfluidic assay to detect SARS-CoV-2 RNA from nasopharyngeal swab samples. Our method leverages isotachophoresis (ITP) to integrate sample preparation, RT-LAMP, and CRISPR-based nucleic acid detection in an automatable chip. For the first time, we use ITP to purify, pre-concentrate and isothermally amplify target nucleic acids into a ~1 µL reaction volume on-chip. The device then transitions LAMP amplicons into an on-chip zone containing Cas12-gRNA complexes and reporter molecules to measure target-activated CRISPR activity. We will use our method to automatically detect COVID-19 from nasopharyngeal swab samples. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.